van Leeuwenhoek Centre for Advanced Microscopy, Amsterdam, The Netherlands
LCAM is a formal collaboration between three innovative microscopy centres at the Faculty of Science (FNWI) of the University of Amsterdam, the Academic Medical Centre (AMC) and the Netherlands Cancer Institute (NKI).
The formalized collaboration capitalizes on a large degree of synergy between the principle investigators in developing, adapting and applying advanced microscopy for use in cell biology. Besides delivering access to the diverse advanced microscopy instruments, LCAM has developed as a leading centre in functional live cell imaging (FRET, FLIM, FCCS, FCS, FRAP and spectral imaging).

Academic Embedding

LCAM-FNWI is embedded within the section of Molecular Cytology and Swammerdam Institute for Life Sciences.

LCAM-AMC is embedded in two research groups of the Department of Cell Biology and Histology: Protein Degradation and Aggregation and DNA Double-strand Break Repair Mechanisms.

LCAM-NKI
consists of the NKI Digital Microscopy Facility, the NKI Robotics and Screening Facility and the Advanced Imaging and Biophysics group.

Principle investigators
FNWI   prof. TWJ Gadella, dr. EMM Manders,
dr. J Goedhart, dr. MA Hink
AMC prof. CJF van Noorden, prof. V Everts,
dr. PM Krawczyk, dr. EAJ Reits,
dr. RA Hoebe, dr. J Stap
NKI dr. K Jalink, dr. J Neefjes, dr. L Oomen,
dr. W Beiersbergen

Microscopy Infrastructure

Highlights

FNWI: Confocal: Zeiss LSM510, Nikon A1 (CLEM, FRAP), Olympus FLIM (FLCCS) Spinning Disk Confocal: Zeiss Yokogawa (FLIM), Nikon Andor (TIRF), Zeiss Yokogawa Widefield: Zeiss Cell Observer, Olympus BX, Nikon CLEM

AMC: Confocal: Leica SP8 X, Leica SP8-SMD (Live-Cells, FLIM, FRAP), Leica SP5-II (multibeam), Nikon C1 (CLEM) Widefield:  Leica (Live Cells), 2x Leica DMR, Leica IRBE (Microinjection) Superresolution: Leica SR-GSD Electron Microscopy: Fei-Technai-12, Philips CM10, Philips 525  (Scanning) Flow Cytometry: 2x BD FacsAria II Cellsorter, BD LSR Fortessa, 2x BD FacscAntoII, BD INflux Cellsorter

NKI: Confocal: 2x Leica SP5-II, Leica SP5 (Live-Cells, spectral, FLIM, FRAP, FCS), 2x Leica Sp5-II (HC Screening) Superresolution: Leica SR-GSD

Education

Courses
- Several beginners (monthly), intermediate and advanced microscopy courses
- International confocal course (30 students/year, 1.5 weeks hands on practical/theoretical course)
- In the footsteps of van Leeuwenhoek international PhD course (16 students/year, 1 week hands on practical/theoretical course)

 Education
- Bachelor Cell Biology & Microscopy course (170 students/year, 12 ECTS)
- Master track in Biomedical Sciences on Cell Biology and Advanced Microscopy (10 students/year, 120 ECTS)
- Several Master student Internships

Lectures
- The Principal Investigators are frequent lecturers and teachers at international (FEBS, EMBO and other) advanced imaging courses.